TssJLM-EM
TssJ-L-M membrane complex
EMDB-2927
Crystal Structures
Research articles and Reviews
2024
Flaugnatti N, Journet L. 2024. Identification of effectors: precipitation of supernatant material. Methods Mol Biol. 2715, 533-538.
PMID: 37930549
Viala JPM, Bouveret E. 2024. Protein-protein interaction: Tandem Affinity Purification in bacteria. Methods Mol Biol. 2715, 285-297.
PMID: 37930536
Zoued A, Duneau JP, Cascales E. 2024. Bacterial One- and Two-Hybrid assays to monitor transmembrane helix interactions. Methods Mol Biol. 2715, 259-271.
PMID: 37930534
Santin YG, Cascales E. 2024. Measure of peptidoglycan degradation activity. Methods Mol Biol. 2715, 197-205.
PMID: 37930529
Chabalier M, Doan T, Cascales E. 2024. Probing protein topology and conformation by limited proteolysis. Methods Mol Biol. 2715, 111-119.
PMID: 37930525
2023
Cascales E. 2023. Crossbow, murder hole, poison and grenade: the deadly arsenal of bacteria. Front Young Minds. 11, 1193114.
PMID: not yet indexed
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Taillefer B, Giraud JF, Cascales E. 2023. No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli. J Bacteriol. 185, e0035723.
PMID: 37971272
Bartoli J, Tempier AC, Guzzi NL, Piras CM, Cascales E, Viala JPM. 2023. Characterization of a (p)ppApp synthetase belonging to a new family of polymorphic toxin associated with temperate phages. J Mol Biol. 435, 168282.
PMID: 37730083
Beauvois SG, Flaugnatti N, Ilbert M, Boyer M, Gavello-Fernandez E, Fronzes R, Jurenas D, Journet L. 2023. The tip protein PAAR is required for the function of the type VI secretion system. Microbiol Spectr. 6, e0147823.
PMID: 37800964
Bourigault Y, Dupont CA, Desjardins JB, Doan T, Bouteiller M, Le Guenno H, Chevalier S, Barbey C, Latour X, Cascales E, Merieau A. 2023. Pseudomonas fluorescens MFE01 delivers a putative type VI secretion amidase that confers biocontrol against the soft-rot pathogen Pectobacterium atrosepticum. Environ Microbiol. 25, 2564-2579.
PMID: 37622480
2023. Qualitative and quantitative methods to measure antibacterial activity resulting from bacterial competition. Bio-Protocol. 13, e4706.
PMID: 37449039
2023. A journey with type IX secretion system effectors: selection, transport, processing and activities. Microbiology. 169, 001320.
PMID: 37043368
2023. Activity and crystal structure of the adherent-invasive Escherichia coli Tle3/Tli3 T6SS effector/immunity complex determined using an AlphaFold2 predicted model. Int J Mol Sci. 24, 1740.
PMID: 36675258
2023. Coevolution-guided mapping of the type VI secretion membrane complex-baseplate interface. J Mol Biol. 435, 167918.
PMID: 36509161
2022
2022. Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop. Nucleic Acids Res. 50, 13114-13127.
PMID: 36484105
2022. The antibacterial type VII secretion system of Baciluus subtilis: structure and interactions of the pseudokinase YukC/EssB. mBio. 13, e0013422.
PMID: 36154281
Song L., Perpich J.D., Wu C.G., Doan T., Nowakowska Z., Potempa J. Christie P.J., Cascales E., Lamont R. Hu B. 2022. A unique bacterial secretion machinery with multiple secretion centers. Proc Natl Acad Sci USA. 119, e2119907119.
PMID: 35471908
This study and the work on the GldLM molecular motor have been featured on the CNRS website (in French): le T9SS, une mitrailleuse à 8 canons !
Vincent M.S., Comas Hervada C., Sebban-Kreuzer C., Le Guenno H., Chabalier M., Kosta A., Guerlesquin F., Mignot T., McBride M.J., Cascales E., Doan T. 2022. Dynamic proton-dependent motors power type IX secretion and gliding motility in Flavobacterium. PLOS Biology. in press
PMID: 35333857
Fuchsbauer O., Lunar Silva I., Cascales E., Roussel A., Leone P. 2022. Structural and functional analyses of the Porphyromonas gingivalis type IX secretion system PorN protein. J Biol Chem. 298, 101618.
PMID: 35065963
Gorasia D.G., Lunar Silva I., Butler C.A., Chabalier M., Doan T., Cascales E., Veith P.D., Reynolds E.C. 2022. Protein interactome analysis of the type IX secretion system identifies PorW as the missing link between the PorK/N ring complex and the Sov translocon. Microbiol. Spectrum. 10, e0160221.
PMID: 35019767
Veith P.D., Glew M.D., Gorasia D.G., Cascales E., Reynolds E.C. 2022. The T9SS system and its role in bacterial function and pathogenesis. J Dent Res. 101, 374-383.
PMID: 34889148
Jurenas D., Cascales E. 2022. T6SS: killing two bugs with one stone. Trends Microbiol. 30, 1-2.
Commentary on Le et al. Proc Natl Acad Sci USA. 118, e2106555118.
PMID: 34753653
2021
Jurenas D., Talachia Rosa L., Rey M. Chamot-Rooke J., Fronzes R., Cascales E. 2021. Mounting, structure and autocleavage of a Type VI secretion-associated Rhs polymorphic toxin. Nat Commun. 12, 6998.
PMID: 34853317
This work has been feratured on the CNRS website (in French): Rhs, une grenade antibactérienne.
Seef S., Herrou J., de Boissier P., My L., Brasseur G., Robert D., Jain R., Mercier R., Cascales E., Habermann B.H., Mignot T. 2021. A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria. eLife. 10, e72409.
PMID: 34505573
Cassán F.D., Coniglio A., Amavizca E., Maroniche G., Cascales E., Bashan Y., de-Bashan L.E. 2021. The Azospirillum brasilense type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana. Environ Microbiol. 23, 6257-6274.
PMID: 34472164
Jurenas D., Payelleville A., Roghanian M., Turnbull K.J., Givaudan A., Brillard J., Hauryliuk V., Cascales E. 2021. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 49, 8384-8395.
PMID: 34255843
Nguyen V.S., Spinelli S., Cascales E., Roussel A., Cambillau C., Leone P. 2021. Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan-binding domain of the TagL accessory protein. PLOS ONE. 16, e0254232.
PMID: 34214145
Lunar Silva I., Cascales E. 2021. Molecular strategies underlying Porphyromonas gingivalis virulence. J. Mol. Biol. 433, 166836.
PMID: 33539891
Jurenas D., Journet L. 2021. Activity, delivery and diversity of type VI secretion effectors. Mol. Microbiol. 115, 383-394.
PMID: 33217073
2020
Flaugnatti N., Rapisarda C., Rey M., Beauvois S.G., Nguyen V.A., Canaan S., Durand E., Chamot-Rooke J., Cascales E., Fronzes R., Journet L. 2020. Structural basis for loading and inhibition of a T6SS phospholipase by the VgrG spike. EMBO J. 39, e104129.
PMID: 32350888
This work has been highlighted on Sarah’s blog on bacterial communication and competition. Click to read !
Brunet Y.R., Bernard C.S., Cascales E. 2020. Fur-Dam regulatory interplay at an internal promoter of the enteroaggregative Escherichia coli Type VI secretion sci1 gene cluster. J Bacteriol. 202, e00075-20.
PMID: 32152218
2019
Santin Y.G. 2019. Uncovering the in vivo proxisome using proximity-tagging methods. Bioessays. 41, e1900131
PMID: 31664723
Santin Y.G., Doan T., Journet L., Cascales E.. 2019. Cell width dictates Type VI secretion tail length. Curr. Biol. 29, 3707-3713
PMID: 31630952
Cherrak Y., Flaugnatti N., Durand E., Journet L., Cascales E.. 2019. Structure and activity of the type VI secretion system. Microbiol. Spectr. 7, PSIB-0031-2019
PMID: 31298206
The T6SS is on the cover of the June issue of Journal of Bacteriology !
Santin Y.G., Camy C.E., Zoued A., Doan T., Aschtgen M.S., Cascales E.. 2019. Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis. J. Bacteriol. 201, e00173-19
PMID: 30910811
The T6SS is on the cover of the May issue of The EMBO Journal !
Rapisarda C., Cherrak Y., Kooger R., Schmidt V., Pellarin R., Logger L., Cascales E., Pilhofer M., Durand E., Fronzes R. 2019. In situ and high-resolution cryo-EM structure of a bacterial Type VI secretion system membrane complex. EMBO J. 38, e100886
PMID: 30877094
2018
Cherrak Y., Rapisarda C., Pellarin R., Bouvier G., Bardiaux B., Allain F. Malosse C., Rey M., Chamot-Rooke J., Cascales E., Fronzes R., Durand E. 2018. Biogenesis and structure of a Type VI secretion baseplate. Nat. Microbiol. 3, 1404-1416 .
PMID: 30323254
see the Behind the paper “Building the blocks of a bacterial weapon” by Chiara.
The T6SS is on the cover of the November issue of Nature Microbiology.
Santin Y.G., Doan T., Lebrun R., Espinosa L., Journet L., Cascales E. 2018. In vivo proximity labelling during Type VI secretion biogenesis reveals TagA as a protein that stops and holds the sheath. Nat. Microbiol. 3, 1304-1313 .
PMID: 30275513
see the Behind the paper “The Type VI secretion system under tension” by Yoann.
see the CNRS press release here
see the News and Views “Tagging the Type VI secretion system” by Olivera Francetic.
Renault M.G., Zamarreno Beas J., Douzi B., Chabalier M. Zoued A., Brunet Y.R., Cambillau C., Journet L., Cascales E. 2018. The gp27-like hub of VgrG serves as adaptor to promote Hcp tube assembly. J. Mol. Biol. 430, 3143-3156.
PMID: 30031895
Zoued A., Duneau J.P., Durand E., Espana A.P., Journet L., Guerlesquin F., Cascales E. 2018. Tryptophan-mediated dimerization of the TssL transmembrane anchor is required for Type VI secretion system activity. J. Mol. Biol. 430, 987-1003.
PMID: 29458124
Chassaing B., Cascales E. 2018. Antibacterial weapons: targeted destruction in the microbiota. Trends Microbiol. 26, 329-338.
PMID: 29452951
Nguyen V.S., Douzi B., Durand E., Roussel A., Cascales E., Cambillau C. 2018. Towards a complete structural deciphering of Type VI secretion system. Curr. Opin. Struct. Biol. 49, 77-84.
PMID: 29414515
Leone P., Roche J., Vincent M.S., Tran Q.H., Desmyter A., Cascales E., Kellenberger C., Cambillau C., Roussel A. 2018. Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasm space. Nat. Commun. 9, 429.
Douzi B., Logger L., Spinelli S., Blangy S., Cambillau C., Cascales E. 2018. Structure-function analysis of the C-terminal domain of the Type VI secretion TssB tail sheath subunit. J. Mol. Biol. 430, 297-309.
2017
Cascales E. 2017. Microbiology: and Amoebophilus invented the machine gun ! Curr Biol. 27, 1170-1173.
see the original article by Böck et al. here
Zoued A., Durand E., Santin Y.G., Journet L., Roussel A., Cambillau C., Cascales E. 2017. TssA: the cap protein of the Type VI secretion system tail. Bioessays. 39, 1600262
Flaugnatti N., Journet L. 2017. Identification of effectors: precipitation of supernatant material. Methods Mol Biol. 1615, 459-464.
Durand E., Lloubès R. 2017. Large Complexes: Cloning Strategy, Production, and Purification. Methods Mol Biol. 1615, 299-309.
Logger L., Zoued A., Cascales E. 2017. Fusion Reporter Approaches to Monitoring Transmembrane Helix Interactions in Bacterial Membranes. Methods Mol Biol. 1615, 199-210.
Santin Y.G., Cascales E. 2017. Measure of Peptidoglycan Hydrolase Activity. Methods Mol Biol. 1615, 151-158.
Vincent M.S., Cascales E. 2017. Probing Inner Membrane Protein Topology by Proteolysis. Methods Mol Biol. 1615, 97-103.
Nguyen V.S., Logger L., Spinelli S., Legrand P., Huyen Pham T.T., Nhung Trinh T.T., Cherrak Y., Zoued A., Desmyter A., Durand E., Roussel A., Kellenberger C., Cascales E., Cambillau C. 2017. Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol. 2, 17103.
see the CNRS press release here
Cascales E. 2017. Inside the chamber of secrets of the Type III secretion system. Cell. 168, 949-951.
see the original article by Hu et al. here
Vincent M.S., Canestrari M.J., Leone P., Stathopulos J., Ize B., Zoued A., Cambillau C., Kellenberger C., Roussel A. & Cascales E. 2017. Characterization of the Porphyromonas gingivalis Type IX secretion trans-envelope PorKLMNP core complex. J Biol Chem. 292, 3252-3261.
2016
Santin Y.G. & Cascales E. 2017. Domestication of a housekeeping transglycosylase for assembly of a Type VI secretion system. EMBO Rep. 18, 138-149.
Douzi B., Brunet Y.R., Spinelli S., Lensi V., Legrand P., Blangy S., Kumar A., Journet L., Cascales E. & Cambillau C. 2016. Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli. Sci Rep. 6, 34405.
The T6SS is on the cover of the November issue of Journal of Molecular Biology !
Zoued A., Cassaro C.J., Durand E., Douzi B., Espana A.P., Cambillau C., Journet L. & Cascales E. 2016. Structure-function analysis of the TssL cytoplasmic domain reveals a new interaction beween the Type VI secretion baseplate and membrane complexes. J Mol Biol. 428, 4413-4423.
Logger L., Aschtgen M.S., Guérin M., Cascales E. & Durand E. 2016. Molecular dissection of the interface between the Type VI secretion TssM cytoplasmic domain and the TssG baseplate component. J Mol Biol. 428, 4424-4437.
Vincent M.S., Durand E. & Cascales E. 2016. The PorX response regulator of the Porphyromonas gingivalis PorXY two-component system does not directly regulate the Type IX secretion genes but binds the PorL subunit. Front Cell Infect Microbiol. 6, 96.
Sana T., Flaugnatti N., Lugo K.A., Lam L.L., Jacobson A., Baylot V., Durand E., Journet L., Cascales E. & Monack D.M. 2016. S. Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sc. USA. 113, 5044-5051.
see the CNRS press release here
This article has been recommended by Eugene Nester on F1000
Zoued A., Durand E., Brunet Y.R., Spinelli S., Douzi B., Guzzo M., Flaugnatti N., Legrand P., Journet L., Fronzes R., Mignot T., Cambillau C. & Cascales E. 2016. Priming and polymerization of a bacterial contractile tail. Nature. 531, 59-63.
PMID: 26909579
see the CNRS press release here
This article has been recommended by Chris Whitfield on F1000
Aussel L., Beuzón C.R., & Cascales E. 2016. Meeting report: adaptation and communication of bacterial pathogens. Virulence. 7, 481-490.
PMID: 26890494
Flaugnatti N., Le TT., Canaan S., Aschtgen MS., Nguyen VS., Blangy S., Kellenberger C., Roussel A., Cambillau C., Cascales E. & Journet L. 2016. A phospholipase A1 antibacterial type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery. Mol Microbiol. 99, 1099-1118.
PMID: 26714038
Journet L., & Cascales E. 2016. The Type VI secretion system in Escherichia coli and related species. EcoSalPlus. 7, 1-20.
2015
Brunet Y.R., Zoued A., Boyer F., Douzi B. & Cascales E. 2015. The Type VI secretion TssEFGK-VgrG phage-like baseplate is recruited to the TssJLM membrane complex via multiple contacts and serves as assembly platform for tail tube/sheath polymerization. PLOS Genetics. 11, e1005545.
Durand E., Nguyen V.S., Zoued A., Logger L., Pehau-Arnaudet G., Aschtgen M.S., Spinelli S., Desmyter A. Bardiaux B., Roussel A., Cambillau C., Cascales E. & Fronzes R. 2015. Biogenesis and structure of a Type VI secretion core membrane complex. Nature. 523, 555-560.
see the CNRS press release here
Brunet Y.R., Khodr A., Logger L., Aussel L., Mignot T., Rimsky S., and Cascales E. 2015. H-NS silencing of the SPI-6-encoded Type VI secretion system limits Salmonella enterica serovar Typhimurium interbacterial killing. Infect Immun. 83, 2738-2750.
Nguyen VS, Logger L., Spinelli S., Desmyter A., Han Lè TT, Kellenberger C., Douzi B., Durand E., Roussel A., Cascales E., and Cambillau C. 2015. Inhibition of Type VI secretion by an anti-TssM llama nanobody. PLOS ONE. 10, e0122187.
Stathopulos J., Cambillau C., Cascales E., Roussel A. and Leone P. 2015. Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis Type IX secretion system. Acta Crystallogr F Struct Biol Commun. 71, 71-74.
Nguyen VS, Spinelli S., Desmyter A., Han Lè T.T., Kellenberger C., Cascales E., Cambillau C. and Roussel A. 2015. Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid antibody. Acta Crystallogr F Struct Biol Commun. 71, 266-271.
2014
Gueguen E., Wills N.M., Atkins J.A., and Cascales E. 2014. Transcriptional frameshiting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genetics. 10, e1004869.
Durand E., Cambillau C., Cascales E., and Journet L. 2014. VgrG, Tae, Tle and beyond: the versatile arsenal of Type VI secretion effectors. Trends Microbiol. 22, 498-507. (Review)
Zoued A., Brunet Y.R., Durand E., Aschtgen M.-S., Logger L., Douzi B., Journet L., Cambillau C., and Cascales E. 2014. Architecture and assembly of the Type VI secretion system. Biochem. Biophys Acta Mol Cell Res. 1843, 1664-1673. (Review)
Douzi B., Spinelli, S., Blangy S., Roussel A., Durand E., Brunet Y.R., Cascales E., and Cambillau C. 2014. Crystal structure and self-interaction of the Type VI secretion tail-tube protein from enteroaggregative Escherichia coli. PLOS ONE. 9, e86918.
Brunet Y.R., Hénin J., Célia H., and Cascales E. 2014. Type VI secretion and bacteriophage tail tubes share a common assembly pathway. EMBO Rep. 15, 315-321.
2013
Zhang X.Y., Brunet Y.R., Logger L., Douzi B., Cambillau C., Journet L. and Cascales E. 2013. Dissection of the TssB-TssC interface during Type VI secretion sheath complex formation. PLOS ONE. 8, e81074.
Zoued A., Durand E., Bebeacua C., Brunet Y.R., Douzi B., Cambillau C., Cascales E. and Journet L. 2013. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane-anchoring complexes of the Type VI secretion system. J. Biol. Chem. 288, 27031-27041.
Gueguen E., Durand E., Zhang X.Y., d’Amalric Q., Journet L. and Cascales E. 2013. Expression of a Yersinia pseudotuberculosis Type VI secretion system is responsive to envelope stresses through the OmpR transcriptional activator. PLOS ONE. 8, e66615.
Brunet Y.R., Espinosa L., Harchouni S., Mignot T. and Cascales E. 2013. Imaging bacterial Type VI secretion-dependent killing. Cell Rep. 3, 36-41.
see the CNRS press release here
Gueguen E. and Cascales E. 2013. Promoter swapping unveils the role of the Citrobacter rodentium CTS1 Type VI secretion system for inter-bacterial competition. Appl. Environ. Microbiol. 79, 32-38.
2012
Durand E., Derrez E., Audoly G., Spinelli S., Ortiz-Lombardia M., Raoult D., Cascales E., and Cambillau C. 2012. Crystal structure of the VgrG1 actin cross-linking domain of the Vibrio cholerae Type VI secretion system. J Biol Chem. 287, 38190-38199.
Silverman J.M., Brunet Y.R., Cascales E., and Mougous J.D. 2012. Structure and regulation of the Type VI secretion system. Ann. Rev. Microbiol. 66, 453-472. (Review)
Durand E., Zoued A., Spinelli S., Watson P.J., Aschtgen M.-S., Journet L., Cambillau C. and Cascales E. 2012. Structural characterization and oligomerization of the TssL protein, a component shared by the bacterial Type VI and Type IVb secretion systems. J. Biol. Chem. 287, 14157-14168.
Aschtgen M.-S., Zoued A., Lloubès R., Journet L., and Cascales E. 2012. The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. MicrobiologyOpen. 1, 71-82.
The T6SS is on the cover of Philosophical Transactions of the Royal Society !
Cascales E. and Cambillau C. 2012. Structural biology of Type VI secretion systems. Phil. Trans. R. Soc. B. 367, 1102-1111. (Review)
2011
Felisberto-Rodrigues C., Durand E., Aschtgen M.S., Blangy S., Ortiz-Lombardia M., Douzi B., Cambillau C. and Cascales E. 2011. Towards a structural comprehension of bacterial Type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathogens. 7, e1002386.
Brunet Y.R., Bernard C.S., Gavioli M., Lloubès R., and Cascales E. 2011. An epigenetic switch involving overlapping Fur and DNA methylation optimizes expression a Type VI secretion gene cluster. PLoS Genetics. 7, e1002205.
Bernard C.S., Brunet Y.R., Gavioli M., Lloubès R., and Cascales E. 2011. Regulation of Type VI secretion gene clusters by 54 and cognate enhancer binding proteins. J Bacteriol. 193, 2158-2167.
2010
Aschtgen M.S., Thomas M.S., and Cascales E. 2010. Anchoring the Type VI secretion system to the cell wall: TssL, TagL, TagP, TagN,… What else? Virulence 1, 535-540.
Aschtgen M.-S., Gavioli M., Dessen A., Lloubès R., and Cascales E. 2010. The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Mol Microbiol. 75, 886-899.
This article has been highlighted in Faculty of 1000 and has been the object of an Addendum in Virulence (Aschtgen et al. Virulence 1, 535-540).
Bernard C.S., Brunet Y.R., Gueguen E., and Cascales E. 2010. Nooks and crannies in Type VI secretion regulation. J Bacteriol. 192, 3850-3860. (Review).
2008
Aschtgen M.-S., Bernard C.S., de Bentzmann S., Lloubès R., and Cascales E. 2008. SciN is an outer membrane lipoprotein required for Type VI secretion in enteroaggregative Escherichia coli. J Bacteriol. 190, 7523-7531.
Cascales E. 2008. The Type VI secretion tool kit. EMBO Rep. 9, 735-741.
