LABO CNRS
  • HOME
  • Lab
    • Environment
    • People
    • Collaborations
  • Themes
    • T6SS
      • Introduction
      • Assembly
      • Function & Effectors
      • Regulation
    • T9SS
  • Funding
  • Publications
    • Structures
    • Research articles and Reviews
  • News
    • Recent news
    • Meetings
  • JOBS
  • Contact
  • Search

Publications

You are here: Home / Publications

Crystal Structures

TssJLM-EM

14 September 2015
TssJ-L-M membrane complex EMDB-2927

TssMJ

14 September 2015
TssM-Cterminus in complex with TssJ PDB 4Y7O

VgrG1

14 September 2015
V. cholerae VgrG1 ACD extension PDB 4E1F

TssJ

0 Comments
/
5 February 2015
TssJ PDB 3RX9

TssL

0 Comments
/
5 February 2015
TssL PDB 3U66

Hcp

0 Comments
/
5 February 2015
Hcp PDB 4HKH

Research articles and Reviews

2022

Song L., Perpich J.D., Wu C.G., Doan T., Nowakowska Z., Potempa J. Christie P.J., Cascales E., Lamont R. Hu B. 2022. A unique bacterial secretion machinery with multiple secretion centers. Proc Natl Acad Sci USA. 119, e2119907119.

PMID: 35471908

see more >

This study and the work on the GldLM molecular motor have been featured on the CNRS website (in French): le T9SS, une mitrailleuse à 8 canons !

Vincent M.S., Comas Hervada C., Sebban-Kreuzer C., Le Guenno H., Chabalier M., Kosta A., Guerlesquin F., Mignot T., McBride M.J., Cascales E., Doan T. 2022. Dynamic proton-dependent motors power type IX secretion and gliding motility in Flavobacterium. PLOS Biology. in press

PMID: 35333857

see more >

Fuchsbauer O., Lunar Silva I., Cascales E., Roussel A., Leone P. 2022. Structural and functional analyses of the Porphyromonas gingivalis type IX secretion system PorN protein. J Biol Chem. 298, 101618.

PMID: 35065963

see more >

Gorasia D.G., Lunar Silva I., Butler C.A., Chabalier M., Doan T., Cascales E., Veith P.D., Reynolds E.C. 2022. Protein interactome analysis of the type IX secretion system identifies PorW as the missing link between the PorK/N ring complex and the Sov translocon. Microbiol. Spectrum. 10, e0160221.

PMID: 35019767

see more >

Veith P.D., Glew M.D., Gorasia D.G., Cascales E., Reynolds E.C. 2022. The T9SS system and its role in bacterial function and pathogenesis. J Dent Res. 101, 374-383.

PMID: 34889148

see more >

Jurenas D., Cascales E. 2022. T6SS: killing two bugs with one stone. Trends Microbiol. 30, 1-2.
Commentary on Le et al. Proc Natl Acad Sci USA. 118, e2106555118.

PMID: 34753653

see more >

2021

Jurenas D., Talachia Rosa L., Rey M. Chamot-Rooke J., Fronzes R., Cascales E. 2021. Mounting, structure and autocleavage of a Type VI secretion-associated Rhs polymorphic toxin. Nat Commun. 12, 6998.

PMID: 34853317

see more >

This work has been feratured on the CNRS website (in French): Rhs, une grenade antibactérienne.

Seef S., Herrou J., de Boissier P., My L., Brasseur G., Robert D., Jain R., Mercier R., Cascales E., Habermann B.H., Mignot T. 2021. A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria. eLife. 10, e72409.

PMID: 34505573

see more >

Cassán F.D., Coniglio A., Amavizca E., Maroniche G., Cascales E., Bashan Y., de-Bashan L.E. 2021. The Azospirillum brasilense type VI secretion system promotes cell aggregation, biocontrol protection against phytopathogens and attachment to the microalgae Chlorella sorokiniana. Environ Microbiol. 23, 6257-6274.

PMID: 34472164

see more >

Jurenas D., Payelleville A., Roghanian M., Turnbull K.J., Givaudan A., Brillard J., Hauryliuk V., Cascales E. 2021. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 49, 8384-8395.

PMID: 34255843

see more >

Nguyen V.S., Spinelli S., Cascales E., Roussel A., Cambillau C., Leone P. 2021. Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan-binding domain of the TagL accessory protein. PLOS ONE. 16, e0254232.

PMID: 34214145

see more >

Lunar Silva I., Cascales E. 2021. Molecular strategies underlying Porphyromonas gingivalis virulence. J. Mol. Biol. 433, 166836.

PMID: 33539891

see more >

Jurenas D., Journet L. 2021. Activity, delivery and diversity of type VI secretion effectors. Mol. Microbiol. 115, 383-394.

PMID: 33217073

see more >

2020

Flaugnatti N., Rapisarda C., Rey M., Beauvois S.G., Nguyen V.A., Canaan S., Durand E., Chamot-Rooke J., Cascales E., Fronzes R., Journet L. 2020. Structural basis for loading and inhibition of a T6SS phospholipase by the VgrG spike. EMBO J. 39, e104129.

PMID: 32350888

see more >

This work has been highlighted on Sarah’s blog on bacterial communication and competition. Click to read !

Brunet Y.R., Bernard C.S., Cascales E. 2020. Fur-Dam regulatory interplay at an internal promoter of the enteroaggregative Escherichia coli Type VI secretion sci1 gene cluster. J Bacteriol. 202, e00075-20.

PMID: 32152218

see more >

2019

Santin Y.G. 2019. Uncovering the in vivo proxisome using proximity-tagging methods. Bioessays. 41, e1900131

PMID: 31664723

see more >

Santin Y.G., Doan T., Journet L., Cascales E.. 2019. Cell width dictates Type VI secretion tail length. Curr. Biol. 29, 3707-3713

PMID: 31630952

see more >

Cherrak Y., Flaugnatti N., Durand E., Journet L., Cascales E.. 2019. Structure and activity of the type VI secretion system. Microbiol. Spectr. 7, PSIB-0031-2019

PMID: 31298206


see more >

The T6SS is on the cover of the June issue of Journal of Bacteriology !

Santin Y.G., Camy C.E., Zoued A., Doan T., Aschtgen M.S., Cascales E.. 2019. Role and recruitment of the TagL peptidoglycan-binding protein during Type VI secretion system biogenesis. J. Bacteriol. 201, e00173-19

PMID: 30910811

see more >

The T6SS is on the cover of the May issue of The EMBO Journal !

Rapisarda C., Cherrak Y., Kooger R., Schmidt V., Pellarin R., Logger L., Cascales E., Pilhofer M., Durand E., Fronzes R. 2019. In situ and high-resolution cryo-EM structure of a bacterial Type VI secretion system membrane complex. EMBO J. 38, e100886

PMID: 30877094

see more >

2018

Cherrak Y., Rapisarda C., Pellarin R., Bouvier G., Bardiaux B., Allain F. Malosse C., Rey M., Chamot-Rooke J., Cascales E., Fronzes R., Durand E. 2018. Biogenesis and structure of a Type VI secretion baseplate. Nat. Microbiol. 3, 1404-1416 .

PMID: 30323254

see more >

see the Behind the paper  “Building the blocks of a bacterial weapon” by Chiara.

The T6SS is on the cover of the November issue of Nature Microbiology.

Santin Y.G., Doan T., Lebrun R., Espinosa L., Journet L., Cascales E. 2018. In vivo proximity labelling during Type VI secretion biogenesis reveals TagA as a protein that stops and holds the sheath. Nat. Microbiol. 3, 1304-1313 .

PMID: 30275513

see more >

see the Behind the paper  “The Type VI secretion system under tension” by Yoann.

see the CNRS press release here

see the News and Views “Tagging the Type VI secretion system” by Olivera Francetic.

Renault M.G., Zamarreno Beas J., Douzi B., Chabalier M. Zoued A., Brunet Y.R., Cambillau C., Journet L., Cascales E. 2018. The gp27-like hub of VgrG serves as adaptor to promote Hcp tube assembly. J. Mol. Biol. 430, 3143-3156.

PMID: 30031895

see more >

Zoued A., Duneau J.P., Durand E., Espana A.P., Journet L., Guerlesquin F., Cascales E. 2018. Tryptophan-mediated dimerization of the TssL transmembrane anchor is required for Type VI secretion system activity. J. Mol. Biol. 430, 987-1003.

PMID: 29458124

see more >

Chassaing B.,  Cascales E. 2018. Antibacterial weapons: targeted destruction in the microbiota. Trends Microbiol. 26, 329-338.

PMID: 29452951

see more >

Nguyen V.S., Douzi B., Durand E., Roussel A., Cascales E., Cambillau C. 2018. Towards a complete structural deciphering of Type VI secretion system. Curr. Opin. Struct. Biol. 49, 77-84.

PMID: 29414515

see more >

Leone P., Roche J., Vincent M.S., Tran Q.H., Desmyter A., Cascales E., Kellenberger C., Cambillau C., Roussel A. 2018. Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasm space. Nat. Commun. 9, 429.

PMID: 29382829

see more >

Douzi B., Logger L., Spinelli S., Blangy S., Cambillau C., Cascales E. 2018. Structure-function analysis of the C-terminal domain of the Type VI secretion TssB tail sheath subunit. J. Mol. Biol. 430, 297-309.

PMID: 29223729

see more >

2017

Cascales E. 2017. Microbiology: and Amoebophilus invented the machine gun ! Curr Biol. 27, 1170-1173.

PMID: 29112872

see more >

see the original article by Böck et al. here

Zoued A., Durand E., Santin Y.G., Journet L., Roussel A., Cambillau C., Cascales E. 2017. TssA: the cap protein of the Type VI secretion system tail. Bioessays. 39, 1600262

PMID: 28817192

see more >

 

Flaugnatti N., Journet L. 2017. Identification of effectors: precipitation of supernatant material. Methods Mol Biol. 1615, 459-464.

PMID: 28667629

see more >

Durand E., Lloubès R. 2017. Large Complexes: Cloning Strategy, Production, and Purification. Methods Mol Biol. 1615, 299-309.

PMID: 28667622

see more >

Logger L., Zoued A., Cascales E. 2017. Fusion Reporter Approaches to Monitoring Transmembrane Helix Interactions in Bacterial Membranes. Methods Mol Biol. 1615, 199-210.

PMID: 28667614

see more >

Santin Y.G., Cascales E. 2017. Measure of Peptidoglycan Hydrolase Activity. Methods Mol Biol. 1615, 151-158.

PMID: 28667610

see more >

Vincent M.S., Cascales E. 2017. Probing Inner Membrane Protein Topology by Proteolysis. Methods Mol Biol. 1615, 97-103.

PMID: 28667606

see more >

Nguyen V.S., Logger L., Spinelli S., Legrand P., Huyen Pham T.T., Nhung Trinh T.T., Cherrak Y., Zoued A., Desmyter A., Durand E., Roussel A., Kellenberger C., Cascales E., Cambillau C. 2017. Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol. 2, 17103.

PMID: 28650463

see more >

see the CNRS press release here

Cascales E. 2017. Inside the chamber of secrets of the Type III secretion system. Cell. 168, 949-951.

PMID: 28283066

see more >

see the original article by Hu et al. here

Vincent M.S., Canestrari M.J.,  Leone P., Stathopulos J., Ize B., Zoued A., Cambillau C., Kellenberger C., Roussel A. & Cascales E. 2017. Characterization of the Porphyromonas gingivalis Type IX secretion trans-envelope PorKLMNP core complex. J Biol Chem. 292, 3252-3261.

PMID: 28057754

see more >

2016

Santin Y.G. & Cascales E. 2017. Domestication of a housekeeping transglycosylase for assembly of a Type VI secretion system. EMBO Rep. 18, 138-149.

PMID: 27920034

see more >

Douzi B., Brunet Y.R.,  Spinelli S., Lensi V., Legrand P., Blangy S., Kumar A., Journet L., Cascales E. & Cambillau C. 2016. Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli. Sci Rep. 6, 34405.

PMID: 27698444

see more >

The T6SS is on the cover of the November issue of Journal of Molecular Biology !

Zoued A., Cassaro C.J., Durand E., Douzi B., Espana A.P., Cambillau C., Journet L. & Cascales E.  2016. Structure-function analysis of the TssL cytoplasmic domain reveals a new interaction beween the Type VI secretion baseplate and membrane complexes. J Mol Biol. 428, 4413-4423.

PMID: 27600409

see more >

Logger L., Aschtgen M.S., Guérin M., Cascales E. & Durand E.  2016. Molecular dissection of the interface between the Type VI secretion TssM cytoplasmic domain and the TssG baseplate component. J Mol Biol. 428, 4424-4437.

PMID: 27600411

see more >

Vincent M.S., Durand E. & Cascales E.  2016. The PorX response regulator of the Porphyromonas gingivalis PorXY two-component system does not directly regulate the Type IX secretion genes but binds the PorL subunit. Front Cell Infect Microbiol. 6, 96.

PMID: 27630829

see more >

Sana T., Flaugnatti N., Lugo K.A., Lam L.L., Jacobson A., Baylot V., Durand E., Journet L., Cascales E. & Monack D.M. 2016. S. Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sc. USA. 113, 5044-5051.

PMID: 27503894

see more >

see the CNRS press release here

This article has been recommended by Eugene Nester on F1000

Zoued A., Durand E., Brunet Y.R., Spinelli S., Douzi B., Guzzo M., Flaugnatti N., Legrand P., Journet L., Fronzes R., Mignot T., Cambillau C. & Cascales E. 2016. Priming and polymerization of a bacterial contractile tail. Nature. 531, 59-63.

PMID: 26909579

see more >

see the CNRS press release here

This article has been recommended by Chris Whitfield on F1000

Aussel L., Beuzón C.R., & Cascales E. 2016. Meeting report: adaptation and communication of bacterial pathogens. Virulence. 7, 481-490.

PMID: 26890494

see more >

Flaugnatti N., Le TT., Canaan S., Aschtgen MS., Nguyen VS., Blangy S., Kellenberger C., Roussel A., Cambillau C., Cascales E. & Journet L. 2016. A phospholipase A1 antibacterial type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery. Mol Microbiol. 99, 1099-1118.

PMID: 26714038

see more >

Journet L., & Cascales E. 2016. The Type VI secretion system in Escherichia coli and related species. EcoSalPlus. 7, 1-20.

PMID: 27223818

see more >

2015

Brunet Y.R., Zoued A., Boyer F., Douzi B. & Cascales E. 2015. The Type VI secretion TssEFGK-VgrG phage-like baseplate is recruited to the TssJLM membrane complex via multiple contacts and serves as assembly platform for tail tube/sheath polymerization. PLOS Genetics. 11, e1005545.

PMID: 26460929

see more >

Durand E., Nguyen V.S., Zoued A., Logger L., Pehau-Arnaudet G., Aschtgen M.S., Spinelli S., Desmyter A. Bardiaux B., Roussel A., Cambillau C., Cascales E. & Fronzes R. 2015. Biogenesis and structure of a Type VI secretion core membrane complex. Nature. 523, 555-560.

PMID: 26200339

see more >

see the CNRS press release here

Brunet Y.R., Khodr A., Logger L., Aussel L., Mignot T., Rimsky S., and Cascales E. 2015. H-NS silencing of the SPI-6-encoded Type VI secretion system limits Salmonella enterica serovar Typhimurium interbacterial killing. Infect Immun. 83, 2738-2750.

PMID: 25916986

see more >

Nguyen VS, Logger L., Spinelli S., Desmyter A., Han Lè TT, Kellenberger C., Douzi B., Durand E., Roussel A., Cascales E., and Cambillau C. 2015. Inhibition of Type VI secretion by an anti-TssM llama nanobody. PLOS ONE. 10, e0122187.

PMID: 25811612

see more >

Stathopulos J., Cambillau C., Cascales E., Roussel A. and Leone P. 2015. Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis Type IX secretion system. Acta Crystallogr F Struct Biol Commun. 71, 71-74.

PMID: 25615973

see more >

Nguyen VS, Spinelli S., Desmyter A., Han Lè T.T., Kellenberger C., Cascales E., Cambillau C. and Roussel A. 2015. Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid antibody. Acta Crystallogr F Struct Biol Commun. 71, 266-271.

PMID: 25760699

see more >

2014

Gueguen E., Wills N.M., Atkins J.A., and Cascales E. 2014. Transcriptional frameshiting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genetics. 10, e1004869.

PMID: 25474156

See more

Durand E., Cambillau C., Cascales E., and Journet L. 2014.  VgrG, Tae, Tle and beyond: the versatile arsenal of Type VI secretion effectors. Trends Microbiol. 22, 498-507. (Review)

PMID: 25042941

See more

Zoued A., Brunet Y.R., Durand E., Aschtgen M.-S., Logger L., Douzi B., Journet L., Cambillau C., and Cascales E. 2014. Architecture and assembly of the Type VI secretion system. Biochem. Biophys Acta Mol Cell Res. 1843, 1664-1673. (Review)

PMID: 24681160

See more

Douzi B., Spinelli, S., Blangy S., Roussel A., Durand E., Brunet Y.R., Cascales E., and Cambillau C. 2014. Crystal structure and self-interaction of the Type VI secretion tail-tube protein from enteroaggregative Escherichia coli. PLOS ONE. 9, e86918.

PMID: 24551044

See more

Brunet Y.R., Hénin J., Célia H., and Cascales E. 2014. Type VI secretion and bacteriophage tail tubes share a common assembly pathway. EMBO Rep. 15, 315-321.

PMID: 24488256

See more

2013

Zhang X.Y., Brunet Y.R., Logger L., Douzi B., Cambillau C., Journet L. and Cascales E. 2013. Dissection of the TssB-TssC interface during Type VI secretion sheath complex formation. PLOS ONE. 8, e81074.

PMID: 24282569

See more

Zoued A., Durand E., Bebeacua C., Brunet Y.R., Douzi B., Cambillau C., Cascales E. and Journet L. 2013. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane-anchoring complexes of the Type VI secretion system. J. Biol. Chem. 288, 27031-27041.

PMID: 23921384

See more

Gueguen E., Durand E., Zhang X.Y., d’Amalric Q., Journet L. and Cascales E. 2013. Expression of a Yersinia pseudotuberculosis Type VI secretion system is responsive to envelope stresses through the OmpR transcriptional activator. PLOS ONE. 8, e66615.

PMID: 23840509

See more

Brunet Y.R., Espinosa L., Harchouni S., Mignot T. and Cascales E. 2013. Imaging bacterial Type VI secretion-dependent killing. Cell Rep. 3, 36-41.

PMID: 23291094

See more

see the CNRS press release here

Gueguen E. and Cascales E. 2013. Promoter swapping unveils the role of the Citrobacter rodentium CTS1 Type VI secretion system for inter-bacterial competition. Appl. Environ. Microbiol. 79, 32-38.

PMID: 23064344

See more

2012

Durand E., Derrez E., Audoly G., Spinelli S., Ortiz-Lombardia M., Raoult D., Cascales E., and Cambillau C. 2012. Crystal structure of the VgrG1 actin cross-linking domain of the Vibrio cholerae Type VI secretion system. J Biol Chem. 287, 38190-38199.

PMID: 22898822

See more

Silverman J.M., Brunet Y.R., Cascales E., and Mougous J.D. 2012. Structure and regulation of the Type VI secretion system. Ann. Rev. Microbiol. 66, 453-472. (Review)

PMID: 22746332

See more

Durand E., Zoued A., Spinelli S., Watson P.J., Aschtgen M.-S., Journet L., Cambillau C. and Cascales E. 2012. Structural characterization and oligomerization of the TssL protein, a component shared by the bacterial Type VI and Type IVb secretion systems. J. Biol. Chem. 287, 14157-14168.

PMID: 22371492

See more

Aschtgen M.-S., Zoued A., Lloubès R., Journet L., and Cascales E. 2012. The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. MicrobiologyOpen. 1, 71-82.

PMID: 22950014

See more

The T6SS is on the cover of Philosophical Transactions of the Royal Society !

Cascales E. and Cambillau C. 2012. Structural biology of Type VI secretion systems. Phil. Trans. R. Soc. B. 367, 1102-1111. (Review)

PMID: 22411981

See more

2011

Felisberto-Rodrigues C., Durand E., Aschtgen M.S., Blangy S., Ortiz-Lombardia M., Douzi B., Cambillau C. and Cascales E. 2011. Towards a structural comprehension of bacterial Type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathogens. 7, e1002386.

PMID: 22102820

See more

Brunet Y.R., Bernard C.S., Gavioli M., Lloubès R., and Cascales E. 2011. An epigenetic switch involving overlapping Fur and DNA methylation optimizes expression a Type VI secretion gene cluster. PLoS Genetics. 7, e1002205.

PMID: 21829382

See more

Bernard C.S., Brunet Y.R., Gavioli M., Lloubès R., and Cascales E. 2011. Regulation of Type VI secretion gene clusters by 54 and cognate enhancer binding proteins. J Bacteriol. 193, 2158-2167.

PMID: 21378190

See more

2010

Aschtgen M.S., Thomas M.S., and Cascales E. 2010. Anchoring the Type VI secretion system to the cell wall: TssL, TagL, TagP, TagN,… What else? Virulence 1, 535-540.

PMID: 21178498

See more

Aschtgen M.-S., Gavioli M., Dessen A., Lloubès R., and Cascales E. 2010. The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Mol Microbiol. 75, 886-899.

PMID: 20487285

See more

This article has been highlighted in Faculty of 1000 and has been the object of an Addendum in Virulence (Aschtgen et al. Virulence 1, 535-540).

Bernard C.S., Brunet Y.R., Gueguen E., and Cascales E. 2010. Nooks and crannies in Type VI secretion regulation. J Bacteriol. 192, 3850-3860. (Review).

PMID: 20511495

See more

2008

Aschtgen M.-S., Bernard C.S., de Bentzmann S., Lloubès R., and Cascales E. 2008. SciN is an outer membrane lipoprotein required for Type VI secretion in enteroaggregative Escherichia coli. J Bacteriol. 190, 7523-7531.

PMID: 18805985

See more

Cascales E. 2008. The Type VI secretion tool kit. EMBO Rep. 9, 735-741.

PMID: 18617888

See more
Eric C’s publications
Laure’s publications
Eric D’s publications

News & events

  • 06 May 20228 May 2022 - 13 h 03 min
  • 28 April 202228 April 2022 - 17 h 27 min
  • 13-21 February 20222 March 2022 - 11 h 14 min
  • 20 March 202226 March 2022 - 11 h 13 min

Publications

  • T9SS cryo-ET26 April 2022 - 10 h 52 min
  • GldLM Motor25 March 2022 - 21 h 39 min
  • T9SS JDR Review21 November 2021 - 16 h 23 min
  • PorW-Sov interaction12 January 2022 - 16 h 21 min

Contact

CNRS – UMR7255
31 Chemin Joseph Aiguier
13402 Marseille Cedex 20
France

Création de sites internet by Quai13.com
  • Twitter
  • WIKI
  • JOBS
  • Contact
Scroll to top